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thiU [2018-05-04 14:55:21]
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thiU [2018-05-04 14:55:21]

thiamine ABC transporter (binding protein)
locus
BSU13240
pI
6.05
mw
21.88 kDa
protein length
200 aa Sequence Blast
gene length
600 bp Sequence Blast
function
thiamine uptake
product
thiamine ABC transporter (binding protein)
essential
no
synonyms
ykoF
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.3|ECF transporter] → [category|SW 1.2.3.3|Class I ECF transporter]
  • [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.2|Biosynthesis of cofactors] → [category|SW 2.6.2.3|Biosynthesis/ acquisition of thiamine]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • Gene

    Coordinates
    1,391,040 → 1,391,642

    The protein

    Structure

  • [PDB|1SBR] (complex with thiamine), [PDB|1S99]

    • [SW|Localization]

  • membrane associated (via [protein|9AF27E4CE34B5C4036840A27D93B13213E3324DD|ThiV]-[protein|236095EDE02EC9EA7768D9D30CED2345D3B7A1A9|ThiX]) [Pubmed|16291685]

    • Expression and Regulation

    Operons

    genes
    [gene|57A953B5D71F3FD8269EAF2366B8E2C7A8C79483|thiU]-[gene|9AF27E4CE34B5C4036840A27D93B13213E3324DD|thiV]-[gene|EF69891D9CF0CE57367B38FB3C69DDE1B689FCB7|thiW]-[gene|236095EDE02EC9EA7768D9D30CED2345D3B7A1A9|thiX]
    description
    [Pubmed|16291685]

    regulatory mechanism

  • [protein|E195900D971AEE4A790D4579BE5E5A15B81010B8|Thi-box]: [SW|RNA switch], via [SW|RNA switch], in [regulon|E195900D971AEE4A790D4579BE5E5A15B81010B8|Thi-box regulon]

    • regulation

  • repressed by thiamine ([protein|search|Thi-box]) [Pubmed|12376536]
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    Biological materials

    Mutant

  • MGNA-A757 (ykoF::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/757 NBRP B. subtilis, Japan]
  • BKK13240 (Δ[gene|57A953B5D71F3FD8269EAF2366B8E2C7A8C79483|thiU]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK13240 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTCTCCGACCTCCTGTT, downstream forward: _UP4_AGAAAAAACAGAAAGCAGGG

    • References

  • 16291685,12376536,15451668